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C:N:P stoichiometry and nutrient limitation of the soil microbial biomass in a grazed grassland site under experimental P limitation or excess

Bryan S Griffiths12*, Annette Spilles13 and Michael Bonkowski3

Author Affiliations

1 Teagasc, Environment Research Centre, Johnstown Castle, Wexford, Co, Wexford, Ireland

2 Current address: SAC, King's Buildings, West Mains Road, Edinburgh, EH9 3JG, UK

3 Mathematisch Naturwissenschaftliche Fakultät der Universität zu Köln, Zoologisches Institut Abt. Terrestrische Ökologie, Biowissenschaftliches Zentrum, Otto-Fischer-Str. 6, D-50674, Köln, Germany

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Ecological Processes 2012, 1:6  doi:10.1186/2192-1709-1-6

Published: 21 June 2012

Abstract

Introduction

The availability of essential nutrients, such as nitrogen (N) and phosphorus (P), can feedback on soil carbon (C) and the soil microbial biomass. Natural cycles can be supplemented by agricultural fertiliser addition, and we determined whether the stoichiometry and nutrient limitation of the microbial biomass could be affected by an unbalanced nutrient supply.

Methods

Samples were taken from a long-term trial (in effect since 1968) with annual applications of 0, 15 and 30 kg P ha−1 with constant N and potassium. Soil and microbial biomass CNP contents were measured and nutrient limitation assessed by substrate-induced respiration. Linear regression and discriminant analyses were used to identify the variables explaining nutrient limitation.

Results

Soil and biomass CNP increased with increasing P fertiliser, and there was a significant, positive, correlation between microbial biomass P and biomass C, apart from at the highest level of P fertilisation when the microbial biomass was over-saturated with P. The molar ratios of C:N:P in the microbial biomass remained constant (homeostatic) despite large changes in the soil nutrient ratios. Microbial growth was generally limited by C and N, except in soil with no added P when C and P were the main limiting nutrients. C, N and P, however, did not explain all the growth limitation on the soils with no added P.

Conclusions

Increased soil C and N were probably due to increased net primary production. Our results confirm that C:N:P ratios within the microbial biomass were constrained (i.e. homeostatic) under near optimum soil conditions. Soils with no added P were characterised by strong microbial P limitation and soils under high P by over-saturation of microorganisms with P. Relative changes in biomass C:P can be indicative of nutrient limitation within a site.

Keywords:
Carbon; Nitrogen; Nutrient limitation; Phosphorus; Soil microbial biomass; Stoichiometry